Coxiella burnetii is a globally dispersed pathogen, causative agent of Q fever, and category B biothreat. Essential to the intracellular lifecycle of Coxiella is the development of a replicative niche known as the Coxiella Containing Vacuole (CCV). To provide a replication-permissive environment, Coxiella secretes an extensive range of effector proteins into the host cell via a type 4 secretion system (T4SS), which interact with and modify a variety of host pathways. In the absence of these effectors Coxiella is incapable of intracellular replication, rendering it non-pathogenic. Despite the identification of ~150 putative effectors, biological roles and mechanisms have been assigned to very few. One unknown effector is CBU2016, which has been shown to be translocated via the T4SS. Our investigations into CBU2016 using knockout (KO) and complimented strains of Coxiella found it did not contribute to bacterial replication, either intracellularly or in axenic media, nor did it alter the pathogenicity of Coxiella in a Galleria mellonella infection model. However, it was observed that CCVs from CBU2016 KO Coxiella were significantly smaller than those from either wild type or complimented strains, while the number of CCVs per infected cell did not differ. Proteomic and western blot analysis of infected HeLa cells identified Disabled-2 (DAB2) as a protein of interest, being present in HeLa cells at lower levels during infection with WT, but not CBU2016 KO Coxiella strains. Transfection experiments also indicated FLAG-tagged CBU2016 expression was associated with decreased cellular DAB2 levels. DAB2 is an adaptor protein involved in clathrin-mediated endocytosis, a mechanism known to be involved in the development of the CCV. However, DAB2 has also been implicated in multiple receptor-mediated signalling pathways, inhibition of Wnt/beta-catenin signalling, cellular differentiation, cell adhesion, and as a tumour suppressor. As such, its key function during Coxiella infection remains unclear. The activity of CBU2016 is therefore of interest not just in the context of Coxiella infection, but for cell biology more generally.